The cryo-EM structure of a bacterial class I transcription activation complex
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6b6h
Q-score: 0.283
Liu B, Hong C, Huang RK, Yu Z, Steitz TA
Science (2017) 358 pp. 947-951 [ Pubmed: 29146813 DOI: doi:10.1126/science.aao1923 ]
- The complex of class-i bacterial transcription activation complex (590 kDa, Cellular component from Escherichia coli)
- Nascent rna 3-mer (1 kDa, RNA from Escherichia coli)
- Synthetic nontemplate strand dna (88-mer) (27 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli O157:H7)
- Dna-directed rna polymerase subunit alpha (8 kDa, Protein from Escherichia coli O157:H7)
- Camp-activated global transcriptional regulator crp (23 kDa, Protein from Escherichia coli O157:H7)
- Synthetic template strand dna (88-mer) (27 kDa, DNA from Escherichia coli)
- Adenosine-3',5'-cyclic-monophosphate (329 Da, Ligand)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli O45:K1)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli O157:H7)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Rna polymerase sigma factor rpod (72 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli O45:K1 (strain S88 / ExPEC))
Focused map on the aCTD-CAP region of the class-I bacterial transcription activation complex
Cryo-EM structure of T. thermophilus 30S Translation Initiation complex
Resolution: 11.5 Å
EM Method: Single-particle
Fitted PDBs: 3j4j
Q-score: 0.057
Simonetti A, Marzi S, Billas IML, Tsai A, Fabbretti A, Myasnikov A, Roblin P, Vaiana AC, Hazemann I, Eiler D, Steitz TA, Puglisi JD, Gualerzi GO, Klaholz BP
PNAS (2013) 110 pp. 15656-15661 [ DOI: doi:10.1073/pnas.1309578110 Pubmed: 24029017 ]
- 30s (850 kDa, Complex from Thermus thermophilus HB8)
- Bacterial 30s translation initiation complex from t. thermophilus containing 30s, if1, if2, mrna and fmet-trna. the structure have been used for modeling full atom if2. (900 kDa, Sample)
- Translation initiation factor 2 (63 kDa, Protein from Thermus thermophilus HB8)
- Fmet-trna (RNA from Escherichia coli)
- Mrna (RNA from synthetic construct)
- Translation initiation factor 1 (8 kDa, Protein from Thermus thermophilus HB8)
Structural insight into nascent polypeptide chain-mediated translational stalling
Resolution: 5.8 Å
EM Method: Single-particle
Fitted PDBs: 4v5h
Q-score: 0.189
Seidelt B, Innis CA, Wilson DN, Gartmann M, Armache JP, Villa E, Trabuco LG, Becker T, Mielke T, Schulten K, Steitz TA, Beckmann R
Science (2009) 326 pp. 1412-1415 [ Pubmed: 19933110 DOI: doi:10.1126/science.1177662 ]
- 70s e. coli (2 MDa, Complex from Escherichia coli)
- Tnac stalled 70s ribosome with p-site trna. (2 MDa, Sample)